Anjul Agarwal
anjul_g@rediffmail.com
International Journal of ChemTech Research
IJCTR
0974-4290
2455-9555
10.20902/CT
http://www.sphinxsai.com/chemtech.php
2019
12
01
Validated RP-HPLC PDA Method for estimation of Trientine Hydrochloride in Pharmaceutical dosage form
P.G.
Sunitha
1
A.
Varalakshmi
2
K.
Rama
3
Department of Pharmaceutical Chemistry, College of Pharmacy, Madras Medical
College, Affiliated to The TamilNaduDr.MGR Medical University, Chennai-03, India
Department of Pharmaceutical Chemistry, College of Pharmacy, Madras Medical
College, Affiliated to The TamilNaduDr.MGR Medical University, Chennai-03, India
Analytical Research and Development, DGM, SaimiraInnopharm Pvt Ltd,
Chennai-98, India
2019
87
92
120109
10.20902/IJCTR.2019.120109
http://dx.doi.org/10.20902/IJCTR.2019.120109
The present work reports a reverse phase high performance liquid chromatography
(RP-HPLC) method for the determination of Trientine hydrochloride in pharmaceutical dosage
form. HPLC was performed using Waters reliant C8 column (250 mm x 4.6 mm ID, 5μm
particle size ) using a mixture of Acetronitrile : Ammonium formate buffer pH 5.3 ± 0.05 as
mobile phase. Ultraviolet detection was carried out at 220 nm. The retention time of Trientine
hydrochloride was found to be 12.497minutes. The developed method was validated as per ICH
guidelines. The proposed method was found to be suitable for the quantification of the selected
drug in pharmaceutical dosage form
http://sphinxsai.com/2019/ch_vol12_no1/1/(87-92)V12N1CT.pdf
RP-HPLC
Validation
Capsule
Trientine hydrochloride
https ://pubchem.ncbi.nlm.niv.gov>com
J Chromatography B
Jun lu, Yi- kam Chan, Sally poppit D, Garsh Cooper JS
859
1
62
2007
J Chromatography B
YukitakaNakavo,Hitoshi Nohka, Hideyuki
774
2
165
2002
Chemical and Pharmaceutical Bulletin
Katsumi Miyazaki, Satoshi Kishino, Michiya Kobayashi
38
4
1035
1990
J Anal Toxicol
Eugene Hansen B,Larry Rushing G,Harold Thompson C
9
4
167
1985
Journal of Chromatography B
Asma Othman, Jun Lu, Tracey Sunderland, Garth CooperJS
860
42
2007
ICH Harmonized tripartite Guideline ICH Q2B, Validation of analytical procedures: Methodology,
May 2007
P.D.Sethi, High performance liquid chromatography quantitative analysis of pharmaceutical
formulations, CBS publishers and distributors, 2001, 105-109
Vogel’s, Text book of quantitative chemical analysis,6th edition ,J.Mendham,RC.Demey JP
Barnes,MJK Thomas Pearson ,2005,289 -315
Ashutosh Kar, Pharmaceutical drug analysis, 2nd edition, New age international private limited, New
Delhi, 2007, 452-466
Beckett A. H., & Stenlake J. B., Practical Pharmaceutical chemistry, (Volume II, 4th edition), CBS
publishers and distributors private limited, New Delhi, 2007, 281-300
Code Q2R1 ICH Guideline, Text on Validation of Analytical Procedure, ICH guidelines, Canada, 2015,
1-16
David Harvey, Modern Analytical Chemistry, 1st edition, A division of the MC Graw hill companies,
Newyork, 2000, 2-6