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International Journal of PharmTech Research CODEN (USA): IJPRIF, ISSN: 0974-4304, ISSN(Online): 2455-9563 Vol.9, No.6, pp 285-290 2016
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Chemical composition of Elamit Scrophularia deserti
Mahnaz Mardani1, Sadegh Rezapour1, Zohreh Eftekhari3,
Majid Asadi-Samani4*, Marzieh Rashidipour5, Omid Afsordeh2, Farzad Kazemzadeh2, Fariba Bahmani2
1Nutritional Health Research Center, Health and Nutritional Department, Lorestan University of Medical sciences, Khorramabad, Iran
2Department of Microbiology, Faculty of Medicine and Clinical Microbiology Research Center, Ilam University of Medical Sciences, Ilam, Iran
3Research & Development Department, Research & Production Complex, Pasteur Institute of Iran, Tehran, Iran
4Medical Plants Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran
4Medical Plants Research Center, Shahrekord University of Medical sciences, Shahrekord, Iran
5Young Researchers and Elite Club, Khorramabad Branch, Islamic Azad University, Khorramabad, Iran
Abstract:Scrophulariadeserti is an annual and perennial herb, as well as a genus of shrubs. Flowers have bilateral or rarely radial symmetry reaching height of 10–50 cm. Leaves of plant are thick, hard, brittle with green color. S. deserti plant from the family of Scrophulariaceae is mostly grass or shrub and rarely trees. I has five-pointed flowers, corolla with lobes and the fruits usually have a capsule with multiple seeds. Aerial parts of Scrophulariadeserti were collected during November of 2014 in Dehloran region of Ilam province. After confirming the species and genius of the plant in natural resources research center of Ilam Province, the collected plant was dried in the air and powdered with mixer. Essential oil was extracted and analyzed with chromatography / mass spectrometry (GC-MS), SMPE method. Phytochemistry results showed that the most active ingredients of the plant were α-PINENE, β-Phellandrene and β-Myrcene which respectively formed 24.69%, 20.58% and 11.82% of the essential oils of the plant.
Keywords: plant, snapdragon, arid, oil, SPME method, α-PINENE.
Introduction
Scrophulariaceae family includes about 268 genera and over 5,100 species. Members of the Scrophulariaceae have a cosmopolitan distribution, with the majority found in high temperate areas, including Arabian Desert and adjacent territory and Touran, including Egypt, Palestine, Jordan, Syria, Iraq, Saudi Arabia, Kuwait, Bahrain and Iran (1). Scrophulariadeserti is annual and perennial herb, as well as a genus of shrubs. Flowers have bilateral or rarely radial symmetry reaching to 10–50 cm height. Leaves of the plant are thick, hard, brittle with green color. S. deserti plant from the family of Scrophulariaceae is mostly grass or shrub and rarely trees, with five-pointed flowers, corolla with lobes and the fruits are usually in a capsule with multiple seeds. (2, 3). This plant is abundant in most parts of Iran and is called Scrophulariadesertisazooei. This plant
has been used in the west region of Iran especially in Ilam province (4). In traditional medicine, local people experimentally use it in some disorders such as internal infections, mastitis, skin ulcers and episiotomy, inflammation, burns, intestinal pain, inflammation, eye and ear infections and hemorrhoids in the form of decoction by oral or topical uses (5,6). Chemical analysis of snapdragon was performed previously, but the plant essential oil chemical analysis has not been reported. In this study, for the first time chemical analysis of essential oil was examined and reported.
Table 1. Scrophularia deserti plant
Species |
Plant family |
Region |
Province |
Latitude |
Longitude |
Altitude (m a.s.l) |
Used part |
Scrophularia deserti |
Scrophulariaceae |
Dehloran city |
Ilam (West of Iran) |
58◦26 N |
13◦33 E |
683-790 |
Aerial organs |
Methods
Preparation of medicinal plants
Specimens of the species of snapdragon were taken from different parts of Ilam provinceduring22 to30 November 2014(Table 1). Oregano plant shoots of 10 samples were collected. From the aerial parts of the plant ten samples were collected. The collected plant was submitted to Natural Resources Research Center of Ilam province and the plant was identified and the essential oils were determined (table 1)
Gas chromatography/mass spectrometry (GC–MS) analysis
The essential oils were analyzed using an Agilent 6890N coupled to Agilent 5973 mass detector gas chromatograph (Agilent, USA) with a HP-5MS 5% phenylmethylsiloxane capillary column (HP-5, 30m (length) × 0.25 mm (ID) × 0.25 µm (stationary phase thickness)). Oven temperature was kept at 55 ◦C for 4 min initially, and then raised at the rate of 4 ◦C/min to 250 ◦C. Injector temperature was set at 250 ◦C, respectively. Helium (99.999%) was used as carrier gas at a flow rate of 0.9 ml/min; samples were injected manually in the split mode. The peaks area percent was used for obtaining quantitative data. The gas chromatograph was coupled to an Agilent 6890N coupled to Agilent 5973 mass detector mass selective detector. Retention indices were calculated for all components using a homologous series of n-alkanes (C5–C24) injected in conditions equal to samples. Identification of oil components was accomplished based on comparison of their retention times with those of authentic standards and by comparison of their mass spectral fragmentation patterns (WILLEY/Chem Station data system)
The characteristics of the instrument were as follow:
Instrument: Gas chromatograph: Agilent 6890N coupled to Agilent 5973 mass detector
Column: HP-5, 30m (length)× 0.25 mm (ID) × 0.25 µm( stationary phase thickness)
Injector type: split/ splitless
Column temperature program:
Rate(⁰C/min) |
Temperature (⁰C) |
Hold (min) |
- |
50 |
- |
5 |
180 |
- |
10 |
250 |
0.00 |
Carrier gas: He (99.999%); Injection type: splitless; Library: Wiley 7n; Injector temperature: 250˚C; Flow rate: 0.9 ml/min
Extraction condition:
Extraction mode: Head space solid phase microextraction (HS-SPME)
SMPE fiber: PDMS 100 µm thickness (SUPELCO)
Sample weight: 0.5 gr, Extraction temperature: 60 ˚C, Extraction time: 15 min, Sonication time: 10 min (Euronda sonication instrument, Italy), Humidity (added water volume): 50 µL, Desorption time in injector port of GC-MS: 3 min.
Results
GC-MS results showed that the Scrophulariadeserti plant contains 51 compounds. Phytochemistry laboratory analyses results are shown in Table 2.
Table 2. Gas chromatography/mass spectrometry (GC–MS) analysis of Scrophulariadeserti
No. |
Compound |
KI |
% |
1 |
α-Thujene |
920 |
0.88 |
2 |
α-PINENE |
931 |
24.69 |
3 |
Sabinene |
966 |
5.81 |
4 |
β-Myrcene |
988 |
11.82 |
6 |
Tricyclene |
997 |
0.94 |
7 |
β-Phellandrene |
1035 |
20.58 |
8 |
3-Carene |
1046 |
0.37 |
9 |
ɤ-Terpinene |
1054 |
0.28 |
10 |
trans-Sabinene hydrate |
1068 |
0.27 |
13 |
Alloocimene |
1176 |
0.75 |
14 |
Camphor |
1224 |
0.68 |
17 |
Fenchyl acetate |
1285 |
2.25 |
20 |
Octadecane |
1289 |
0.18 |
21 |
Borneol, acetate |
1296 |
2.98 |
27 |
Bicycloelemene |
1325 |
1.14 |
30 |
α-Copaene |
1378 |
2.24 |
31 |
Calarene |
1386 |
1.48 |
32 |
β- BOURBONENE |
1392 |
0.26 |
35 |
α-Gurjunene |
1406 |
0.29 |
37 |
trans-Caryophyllene |
1418 |
9.22 |
39 |
α-Bergamotene |
1436 |
1.03 |
40 |
Isoledene |
1449 |
0.49 |
41 |
trans-β-Farnesene |
1468 |
3.14 |
43 |
α-amorphene |
1474 |
0.40 |
44 |
β-Cubebene |
1485 |
2.38 |
46 |
bicyclogermacrene |
1492 |
1.57 |
47 |
β-Bisabolene |
1512 |
0.61 |
48 |
Germacrene D |
1526 |
1.15 |
49 |
δ-Cadinene |
1538 |
1.88 |
51 |
Docosane |
1568 |
0.25 |
Discussion
The results of a phytochemical study showed that, α-PINENE with 24.69% is the most active ingredient of Scrophularia esert plant. Phytochemical analysis revealed that Scrophularia eserti xtracts containing compounds 3 (zeta) -hydroxy-octadeca-4 (E), 6 (Z) -dienoic acid (1). The known compounds are ajugoside (2), scropolioside B (3), 6-O-alpha-L-rhamnopyranosylcatalpol (4), buddlejoside A (8) (5), scrospioside A (6), laterioside and 3R-1 -octan-3-yl-3-O-beta-D-glucopyranoside (7). Scrophularia eserti is a plant which in traditional medicine, local people experimentally use it in internal infections, mastitis, skin ulcers and episiotomy, inflammation, burns, intestinal pain, inflammation, eye and ear infections and hemorrhoids in the form of decoction by oral or topical uses (5,6). This plant has phenolic compounds and phenolic compounds have antimicrobial activities (8-13). More importantly these compounds have antioxidant activities which
possess various therapeutic effects, especially in diabetes (14-17), hyperlipidemia (18-20), renal toxicities (21-35), pain (36-39) and neurological diseases (40-45). Therefore, this plant might have these properties, too. There are many therapeutic effects of medicinal plants due to pharmaceutical active substances such as phenols, flavonoids, tannins, anthocyanin and etc (46-55).
References
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