Anjul Agarwal
anjul_g@rediffmail.com
International Journal of ChemTech Research
IJCTR
0974-4290
2455-9555
10.20902/CT
http://www.sphinxsai.com/chemtech.php
2019
12
01
Assay of Lipase and Biosurfactant Production Activity of Two Keratinolytic BacteriaAeromonas media LU04 and Enterobacter tabaci PK09
Yayuk Putri
Rahayu
1
Dwi
Suryanto
1
Erman
Munir
1
Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas
Sumatera Utara, Jln. Bioteknologi No. 1, Medan 20155, Indonesia
2019
121
128
120114
10.20902/IJCTR.2019.120114
http://dx.doi.org/10.20902/IJCTR.2019.120114
Assay of lipase and biosurfactant productionactivity of keratinolytic bacterial
isolateshas been conducted. Bacterial isolates was collection of Microbiology
Laboratory,Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas
Sumatera Utara, Medan, Indonesia. Assay on lipase activity was done qualitatively using
sensitive plate assay method using rhodamine-B olive oil agar and quantitatively using copper
soap colorimetric method with cuppric acetate-pyridine reagent and olive oil as substrate.
Characterization of lipase was performed in different pH and temperature. Biosurfactant
production assay was conducted using oil displacement method to measure oil displacement
area (ODA) and measured emulsification index (EI24).The two potential bacteria Aeromonas
media LU04 andEnterobacter tabaci PK09 out of four bacterial isolates showed higher lipase
activity with produced more orange fluorescence around the colony with lipase activity of3.767
and 2.054 U/mL and specific activity were 0.443 and 0.346 U/mg, respectively. The lipase
activity of both bacteria was optimum at pH 7 and at temperature of 40oC. Biosurfactant
production activity showed ODA value of 38.0 and 15.0 mm, and with emulsification index of
20 and 15%. Biosurfactant activity was in line with lipase activity. This might indicated that
biosurfactant prodcution and activity was needed in lipase activity
http://sphinxsai.com/2019/ch_vol12_no1/1/(121-128)V12N1CT.pdf
biosurfactant
keratinolytic bacteria
lipase
Aeromonas media
Enterobacter tabaci
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