Anjul Agarwal
anjul_g@rediffmail.com
International Journal of PharmTech Research
IJPTR
0974-4304
2455-9563
10.20902/2017
http://www.sphinxsai.com/pharmtech.php
2018
11
4
Stability Indicating Thin-Layer Chromatographic Determination of Brivarecetam as Bulk Drug: Application to Forced Degradation Study
Shital R.
Nikhar
1
Deepali A.
Bansode
1
Department of Pharmaceutical Chemistry& Department of Quality Assurance
Techniques, Bharati Vidyapeeth University, Poona College of Pharmacy, Pune,
Maharashtra, India-411038
2018
351
360
11406
10.20902/IJPTR.2018.11406
http://dx.doi.org/10.20902/IJPTR.2018.11406
A new, accurate, selective, simple and precise HPTLC method for estimation of
briveracetam in bulk drug as well as tablet dosage form was developed and validated. The drug
was well separated using mobile phase of ammonium acetate: methanol:n-propanol (8:1.6:1.6
v/v/v) with densitometric quantification of brivaracetam at 242nm. The TLC parameters were
standardized and the Rfof brivaracetam was determined to be 0.40. The values of Linearity
(200-1200ng/spot), Method precision (intra-day RSD 0.5-0.8% and inter-day RSD 0.25-
0.46%), Accuracy (% recovery 97.53%-102.84%)and specificity were determined according to
ICH guidelines. Brivaracetam was exposed to various stress condition to study degradation
profile. Degradation was seen in acidic, basic and oxidative condition. Brivaracetam was found
to be stable in photolytic condition. The experiment gives us satisfactory result for method
validation and development indicates the successful validation of HPTLC method for
quantitative determination of brivaracetam. The HPTLC method is simple, rapid, economic
andmore suitable for routine analysis of brivaracetam in bulk and tablet dosage forms
http://www.sphinxsai.com/2018/ph_vol11_no4/1/(351-360)V11N04PT.pdf
Brivaracetam
HPTLC
Densitometric estimation
Method development
Validation
Stability indicating method
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