Anjul Agarwal
anjul_g@rediffmail.com
International Journal of ChemTech Research
IJCTR
0974-4290
2455-9555
10.20902/CT
http://www.sphinxsai.com/chemtech.php
2018
11
10
Biofilm Formation and Multiple Antibiotic Resistance Index of Bacteria Isolates from Saliva, Teeth and Necrotic Roots Canals of Teeth of Dental Patients
Merriam Ghadhanfar
Alwan
1
Ashraf Abbas
Drais
1
Asmat
Ahmad
1
School of Bioscience and Biotechnology,Faculty of Science and Technology,
UniversitiKebangsaan Malaysia, 43600 Bangi, Selangor, Malaysia
2018
84
94
111012
10.20902/IJCTR.2018.111012
http://dx.doi.org/10.20902/IJCTR.2018.111012
Globally, antibiotic resistance among oral microbiota has constituted an increasing
health challenge, and limited information regarding such resistance is available. This study
was designed to isolate both aerobic and anaerobic bacteria from different sources of oral
samples and screen the isolates for biofilm forming and antibiotics resistance abilities. A total
of 72 samples were collected, 21, 31 and 20 were from saliva, teeth and necrotic roots canals
respectively. In general, among 267 total isolates, 16.2% were identified as Enterococcus sp.
and it was considered as the most prevalence genus, followed by Streptococcus sp. (15.8),
Staphylococcus sp. (13.5%), E. coli (7.5%), Bacillus sp. (6.4%), Enterobacter sp. (5.6%),
Pseudomonas sp. (4.5%), Proteus sp. (4.5%), Clostridium sp. (4.1%), Actinomyces sp. (3%),
Peptostreptococcus sp. (3%), Klebsiella sp. (2.6%), Bacteroides sp. (2.6%), Lactobacillus sp.
(2.3%) , Fusobacterium sp. (1.9%), Micrococcs sp. (1.5%), Salmonella sp. (1.1%), Provotella
sp. (1.1%), Shigella sp. (0.8%), Eubacterium sp. (0.8%), Aerococcus sp. (0.8%),
Chromobacterium sp. (0.4%).Three methods were used to detect the biofilm formation ability
of the isolates. The results showed that the percentages of strong biofilm formation of the
isolates for each method were 11.9%, 27.7%and 39% for Congo red agar method, Tube
method and Microtitre plate methodrespectively. The highest multiple antibiotic resistance
index (MAR)was among the isolates from necrotic roots canals (0.82) followed by teeth (0.71)
and saliva (0.69). According to isolate’s genera, Enterococcus sp. showed the highest MAR
indices among the isolates, which recorded 0.97, 0.96, and 0.89 for the isolates from saliva,
necrotic roots canals and teeth respectively
http://sphinxsai.com/2018/ch_vol11_no10/1/(84-94)V11N10CT.pdf
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